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Extraction procedure: Whenever possible, systemically infected leaf material, freshly harvested, should be the source of choice for virus purification. Virus particles are extracted by crushing infected leaves in a suitable extraction medium at 4°C with a commercially available food blender.
Grind g leaf material in liq. N2 to fine powder using prechilled pestle and mortar. 3. Transfer the powder to 15 ml polypropylene tubes containing ml of prewarmed extraction buffer.
Profiling bacterial transcriptome in planta is challenging due to the low abundance of bacterial RNA in infected plant tissues. Here, we describe a protocol to profile transcriptome of a foliar bacterial pathogen, Pseudomonas syringae pv. tomato DC3000, in the leaves of Arabidopsis thaliana at an early stage of infection using RNA sequencing (RNASeq).
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Leaf color was measured weekly using a SPAD chlorophyll meter (SPAD 502, Minolta Co, LTD, Japan) between 6 March and 1 May 2008. Three upper leaflets from different areas of each pot canopy were measured and the average recorded.
Vortex mixer (Scientific Industries, model: VortexGenie 2, catalog number: SI0236) ... Ensure that the samples do not thaw during crushing. Figure 2. Crushed leaf sample before incubation in the bacterial isolation buffer; ... into a new ml Eppendorf tube by pipetting.
THE COUNCIL OF THE EUROPEAN COMMUNITIES, Having regard to the Treaty establishing the European Economic Community, and in particular Article 43 thereof, Having regard to the propo
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Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot. Commission Directive 2006/56/EC of 12 June 2
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